Eye Piece

1. Interpupillary distance adjustment
   1. While looking through the eyepieces with a sample in focus, adjust for binocular vision until the left and right fields of view coincide completely.  You should see one clear image when properly adjusted, out of adjustment and you will see double images.
2. Diopter Adjustment
   1. Looking through the right eyepiece (2) with your right eye, rotate the course and fine adjustment knobs to bring the specimen into focus.
   2. Looking Turn the diopter adjustment rings on both eyepieces to align their engraved lines with the edge of the outer tub of the eyepiece.  This is the standard position.
   3. Focus on the sample with the 10X objective using bright-field.
   4. Bring the 50X objective into the optical path and focus on the sample by turning the coarse/fine focus knobs.
   5. Bring the 5x or 10x objective into the optical path and focus on the sample by turning the diopter ring on the right eyepiece (not the coarse/fine adjustment knobs). 
   6. Now looking through the left eyepiece with your left eye, turn focus the sample by turing the left diopter adjustment ring (1) to focus on the specimen.
3. Eye Shades
   1. Since you will be using safety glasses of some sort, use with the eye shades in their normal folded down position. This will prevent the eyeglasses from being scratched.

1. 1. .
   2. Repeat for all objectives, making small adjustments to the diopter rings until the image stays in focus using any objective.

BF/DF Light Path Selection

1. Slide the mirror selector lever (1) toward the indication of the mirror for the desired observation method.
   1. BF: Reflected light brightfield observation
   2. DF: Reflected light darkfield observation

• Be sure to slide the mirror selector lever until it contacts the stopper position.

Effects of Dummy Slider -The dummy slider (2) has been installed on the revolving nosepiece at the factory. It can be replaced by loosening the clamping knob (3) when a DIC prism is to be used. However, in observations other than DIC, be sure to push in the dummy slider in order for prevention of flare.

Filters

ND Filter Knob - The ND filter is interlocked with the brightfield (BF) light path switching so it can be engaged or disengaged according to the mirror selector lever (1). The ND filter makes it possible to reduce the glare when darkfield (DF) is switched to brightfield (BF).

Filter Slots - Engage the optimum filter sliders for the purpose of observation in the two filter insertion slots (3). Be sure to engage from the left side. The first click position is the idle position and the second click engages the filter in the light path.

Field Iris

The field iris is used to restrict the diameter of the beam of light entering the objective and thus excludes stray light from the observation path improving image contrast.  The diameter of the field iris should be adjusted for each objective power to the extent that is just circumscribes the field of view.

The field iris diaphragm knob (2) and the field iris centering screw (3) are used to adjust the field iris size and position in relation to the observation path.

Aperture Iris

The aperture iris diaphragm determines the numerical aperture of the illumination system. Matching the numerical aperture of the illumination system with that of the objective provides better image resolution and contrast, and also increases the depth of focus.

The aperture iris diaphragm knob (2) and the aperture iris centering knob (3) are used to adjust the aperture iris size and position in relation to the observation path.

Focus Knob

1. Coarse adjustment knob
   1. The larger knob is course stage adjustment and is used to quickly move the stage up and down for sample focusing.
2. Fine adjustment knob - Shown installed on the left side in this illustration, actual installation on the right side at Birck.
   1. The small knob farthest from the microscope base is the fine adjustment knob, and is used to move the stage vertically in small increments to facilitate fine focus of the sample.
3. Coarse adjustment knob tension adjustment (1).
   1. Typically, the coarse adjustment knob tension is preadjusted for easy use.
   2. If adjusted, turning in the direction of the arrow will increase tension and vise versa.
   3. If the stage drops by itself when the knob is released, or the microscope quickly loses focus after adjustment the tension is set too low, rotate the ring in the direction of the arrow to correct.

XY Axis Knob

1. Y axis translation knob is the top knob and rotation will cause the stage to translate toward and away from you when looking in the eyepiece.
2. X axis translation knob is the bottom knob and rotation will cause the stage to translate left and right when looking in the eyepiece.
3. Y axis lock (3) is used to lock the y axis, and only allow translation in the x direction.

Coarse Focus Stopper

The coarse focus stopper restricts the movement of the coarse focus knob so the stage cannot be raised beyond the operators specified limit.  

With the sample in focus, turn the coarse focus stopper ring as far as it will go in the direction of the arrow on the base of the microscope (about 3/4 revolution).  The coarse focus stopper is now clamped in position.  When changing the sample, lower the stage by turning only the coarse focus knob.  After changing the sample, gently raise the stage by turning only the coarse focus knob as far as it will go.  The sample should be roughly in focus when the stage has been raised as far as it will go.  Use the fine focus knob to bring the sample into perfect focus.