The JEOL system is calibrated by staff every 7 - 21 days, with separate calibration necessary for each condition file (current). These are typically stable for 1-3 weeks. As part of this process, column shift and tilt is adjusted (similar to an SEM), current is measured (and adjusted via the column zoom lenses, if necessary), wobble is checked and minimized (via the objective aperture, again similar to an SEM), focus and astigmatism is adjusted (via the objective lens strength and stigmator correction values), and the DAILYCAL file is run to ensure everything passes. Some particularly relevant measurements are shown below: Current: specified as nominal values. As part of the exposure, the machine will measure the current (by default, every 20 minutes) and automatically adjust the exposure dose based on this. As such, drifts in current will not directly affect the writing quality, but may affect user experience on the system. Calibration measured beam size: The beam size in the X and Y directions measured by the system on a “clean” spot of the AE mark used by staff for calibration. This will always be larger than the true size of the beam, and is only useful in the context explained here.
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