iLab Name: Bruker Catalyst AFM
iLab Kiosk: BRK Metrology Core
FIC: Helen McNally
Owner: Joon Park
Location: BRK 1265
Maximum Wafer Size: 

Overview

General Description

The BioScope Catalyst Life Science AFM has been designed from top to bottom to make it easier to realize the benefits of combining atomic force microscopy and light microscopy.

Specifications

• X/Y range: 150 microns
• Z range: 23 microns 
• XY sample stage: motorized stage with 10 x 10 mm range, includes magnetic sample clamps for 1x 3in slides, 25mm coverslips, 35 and 60mm plastic petri dishes, and 50mm glass bottom petri dishes.
• Height noise: <0.1nm RMS (air); <0.2nm RMS (fluid)
• Force noise: thermally-limited, PicoForce-quality force measurements, <10pN RMS for cantilever with k=20pN/nm
• Inverted optical microscopes: Olympus IX71
• AFM controller: NanoScope V

Sample Requirements and Preparation

Standard Operating Procedure

Questions & Troubleshooting

References

Bruker Catalyst AFM User's Guide (Internal Resource)

Bruker Catalyst AFM Experiment Guide (Internal Resource)

Measuring the Elastic Properties of Living Cells with Atomic Force Microscopy Indentation by JL MacKay and S Kumar (2013) (Internal Resource)

Bruker(prev. Veeco) Catalyst introduction: https://youtu.be/VS7o6JIqJAM, https://youtu.be/fFkr3RTr-G0
Catalyst AFM training video from Western Washington University
   1. AFM demo/instrument: https://youtu.be/Jcze3sMc4Q4
   2. AFM demo/software: https://youtu.be/I7jbC8kxwZA
   3. Theory and background: https://youtu.be/VWDS-PiKOL8
   4. Operation: https://youtu.be/2qZO-aXjW18